Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-176_S15_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-176_S15_L003_R2_001_val_2.fq.gz

Data processed at 05:39 on 2019-12-21


Alignment Stats

Sequence pairs analysed in total 36345555
Paired-end alignments with a unique best hit 13724724
Pairs without alignments under any condition 19810124
Pairs that did not map uniquely 2810707
Genomic sequence context not extractable (edges of chromosomes) 7

Cytosine Methylation

Total C's analysed 415508402
Methylated C's in CpG context 15449887
Methylated C's in CHG context 1795361
Methylated C's in CHH context 4293575
Methylated C's in Unknown context 73998
Unmethylated C's in CpG context 44600919
Unmethylated C's in CHG context 86098830
Unmethylated C's in CHH context 263269830
Unmethylated C's in Unknown context 887032
Percentage methylation (CpG context) 25.7%
Percentage methylation (CHG context) 2.0%
Percentage methylation (CHH context) 1.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6759192 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 6965525 original bottom strand

Deduplication

Alignments analysed 13724717
Unique alignments 10094686
Duplicates removed 3630031
Duplicated alignments were found at 2189122 different positions

Cytosine Methylation after Extraction

Total C's analysed 212732771
Methylated C's in CpG context 8409431
Methylated C's in CHG context 937010
Methylated C's in CHH context 2366691
Unmethylated C's in CpG context 21389527
Unmethylated C's in CHG context 42655847
Unmethylated C's in CHH context 136974265
Percentage methylation (CpG context) 28.2%
Percentage methylation (CHG context) 2.1%
Percentage methylation (CHH context) 1.7%

M-Bias Plot