Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-44_S41_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-44_S41_L005_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 662
Pairs without alignments under any condition 99338
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 22316
Methylated C's in CpG context 61
Methylated C's in CHG context 289
Methylated C's in CHH context 87
Methylated C's in Unknown context 4
Unmethylated C's in CpG context 6526
Unmethylated C's in CHG context 6229
Unmethylated C's in CHH context 9124
Unmethylated C's in Unknown context 2
Percentage methylation (CpG context) 0.9%
Percentage methylation (CHG context) 4.4%
Percentage methylation (CHH context) 0.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 470 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 192 original bottom strand

Deduplication

Alignments analysed 662
Unique alignments 548
Duplicates removed 114
Duplicated alignments were found at 66 different positions

Cytosine Methylation after Extraction

Total C's analysed 11969
Methylated C's in CpG context 30
Methylated C's in CHG context 140
Methylated C's in CHH context 51
Unmethylated C's in CpG context 3484
Unmethylated C's in CHG context 3241
Unmethylated C's in CHH context 5023
Percentage methylation (CpG context) 0.9%
Percentage methylation (CHG context) 4.1%
Percentage methylation (CHH context) 1.0%

M-Bias Plot