Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-43_S40_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-43_S40_L005_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 841
Pairs without alignments under any condition 99159
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 29803
Methylated C's in CpG context 102
Methylated C's in CHG context 354
Methylated C's in CHH context 94
Methylated C's in Unknown context 3
Unmethylated C's in CpG context 8642
Unmethylated C's in CHG context 8293
Unmethylated C's in CHH context 12318
Unmethylated C's in Unknown context 13
Percentage methylation (CpG context) 1.2%
Percentage methylation (CHG context) 4.1%
Percentage methylation (CHH context) 0.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 583 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 258 original bottom strand

Deduplication

Alignments analysed 841
Unique alignments 727
Duplicates removed 114
Duplicated alignments were found at 62 different positions

Cytosine Methylation after Extraction

Total C's analysed 16624
Methylated C's in CpG context 52
Methylated C's in CHG context 205
Methylated C's in CHH context 58
Unmethylated C's in CpG context 4807
Unmethylated C's in CHG context 4570
Unmethylated C's in CHH context 6932
Percentage methylation (CpG context) 1.1%
Percentage methylation (CHG context) 4.3%
Percentage methylation (CHH context) 0.8%

M-Bias Plot