Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-41_S38_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-41_S38_L005_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 617
Pairs without alignments under any condition 99383
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 22262
Methylated C's in CpG context 66
Methylated C's in CHG context 257
Methylated C's in CHH context 106
Methylated C's in Unknown context 9
Unmethylated C's in CpG context 6651
Unmethylated C's in CHG context 6218
Unmethylated C's in CHH context 8964
Unmethylated C's in Unknown context 4
Percentage methylation (CpG context) 1.0%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 1.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 447 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 170 original bottom strand

Deduplication

Alignments analysed 617
Unique alignments 539
Duplicates removed 78
Duplicated alignments were found at 43 different positions

Cytosine Methylation after Extraction

Total C's analysed 12164
Methylated C's in CpG context 37
Methylated C's in CHG context 136
Methylated C's in CHH context 60
Unmethylated C's in CpG context 3620
Unmethylated C's in CHG context 3380
Unmethylated C's in CHH context 4931
Percentage methylation (CpG context) 1.0%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 1.2%

M-Bias Plot