Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-229_S36_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-229_S36_L004_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 706
Pairs without alignments under any condition 99294
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 25788
Methylated C's in CpG context 64
Methylated C's in CHG context 359
Methylated C's in CHH context 92
Methylated C's in Unknown context 3
Unmethylated C's in CpG context 7416
Unmethylated C's in CHG context 7161
Unmethylated C's in CHH context 10696
Unmethylated C's in Unknown context 10
Percentage methylation (CpG context) 0.9%
Percentage methylation (CHG context) 4.8%
Percentage methylation (CHH context) 0.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 471 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 235 original bottom strand

Deduplication

Alignments analysed 706
Unique alignments 628
Duplicates removed 78
Duplicated alignments were found at 54 different positions

Cytosine Methylation after Extraction

Total C's analysed 14593
Methylated C's in CpG context 36
Methylated C's in CHG context 199
Methylated C's in CHH context 61
Unmethylated C's in CpG context 4152
Unmethylated C's in CHG context 3985
Unmethylated C's in CHH context 6160
Percentage methylation (CpG context) 0.9%
Percentage methylation (CHG context) 4.8%
Percentage methylation (CHH context) 1.0%

M-Bias Plot