Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-227_S35_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-227_S35_L004_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 393
Pairs without alignments under any condition 99607
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 14367
Methylated C's in CpG context 59
Methylated C's in CHG context 207
Methylated C's in CHH context 57
Methylated C's in Unknown context 7
Unmethylated C's in CpG context 4210
Unmethylated C's in CHG context 3866
Unmethylated C's in CHH context 5968
Unmethylated C's in Unknown context 1
Percentage methylation (CpG context) 1.4%
Percentage methylation (CHG context) 5.1%
Percentage methylation (CHH context) 0.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 270 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 123 original bottom strand

Deduplication

Alignments analysed 393
Unique alignments 368
Duplicates removed 25
Duplicated alignments were found at 22 different positions

Cytosine Methylation after Extraction

Total C's analysed 8619
Methylated C's in CpG context 34
Methylated C's in CHG context 118
Methylated C's in CHH context 36
Unmethylated C's in CpG context 2515
Unmethylated C's in CHG context 2277
Unmethylated C's in CHH context 3639
Percentage methylation (CpG context) 1.3%
Percentage methylation (CHG context) 4.9%
Percentage methylation (CHH context) 1.0%

M-Bias Plot