Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-226_S34_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-226_S34_L004_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 321
Pairs without alignments under any condition 99679
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 12469
Methylated C's in CpG context 26
Methylated C's in CHG context 152
Methylated C's in CHH context 66
Methylated C's in Unknown context 4
Unmethylated C's in CpG context 3625
Unmethylated C's in CHG context 3431
Unmethylated C's in CHH context 5169
Unmethylated C's in Unknown context 4
Percentage methylation (CpG context) 0.7%
Percentage methylation (CHG context) 4.2%
Percentage methylation (CHH context) 1.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 221 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 100 original bottom strand

Deduplication

Alignments analysed 321
Unique alignments 298
Duplicates removed 23
Duplicated alignments were found at 16 different positions

Cytosine Methylation after Extraction

Total C's analysed 7486
Methylated C's in CpG context 16
Methylated C's in CHG context 85
Methylated C's in CHH context 41
Unmethylated C's in CpG context 2160
Unmethylated C's in CHG context 2046
Unmethylated C's in CHH context 3138
Percentage methylation (CpG context) 0.7%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 1.3%

M-Bias Plot