Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-220_S32_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-220_S32_L004_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 409
Pairs without alignments under any condition 99591
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 15161
Methylated C's in CpG context 59
Methylated C's in CHG context 153
Methylated C's in CHH context 87
Methylated C's in Unknown context 4
Unmethylated C's in CpG context 4243
Unmethylated C's in CHG context 4218
Unmethylated C's in CHH context 6401
Unmethylated C's in Unknown context 3
Percentage methylation (CpG context) 1.4%
Percentage methylation (CHG context) 3.5%
Percentage methylation (CHH context) 1.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 280 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 129 original bottom strand

Deduplication

Alignments analysed 409
Unique alignments 380
Duplicates removed 29
Duplicated alignments were found at 19 different positions

Cytosine Methylation after Extraction

Total C's analysed 9032
Methylated C's in CpG context 31
Methylated C's in CHG context 84
Methylated C's in CHH context 49
Unmethylated C's in CpG context 2523
Unmethylated C's in CHG context 2458
Unmethylated C's in CHH context 3887
Percentage methylation (CpG context) 1.2%
Percentage methylation (CHG context) 3.3%
Percentage methylation (CHH context) 1.2%

M-Bias Plot