Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-215_S31_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-215_S31_L004_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 689
Pairs without alignments under any condition 99311
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 23984
Methylated C's in CpG context 80
Methylated C's in CHG context 340
Methylated C's in CHH context 102
Methylated C's in Unknown context 8
Unmethylated C's in CpG context 7002
Unmethylated C's in CHG context 6800
Unmethylated C's in CHH context 9660
Unmethylated C's in Unknown context 5
Percentage methylation (CpG context) 1.1%
Percentage methylation (CHG context) 4.8%
Percentage methylation (CHH context) 1.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 494 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 195 original bottom strand

Deduplication

Alignments analysed 689
Unique alignments 604
Duplicates removed 85
Duplicated alignments were found at 52 different positions

Cytosine Methylation after Extraction

Total C's analysed 13319
Methylated C's in CpG context 48
Methylated C's in CHG context 175
Methylated C's in CHH context 54
Unmethylated C's in CpG context 3846
Unmethylated C's in CHG context 3720
Unmethylated C's in CHH context 5476
Percentage methylation (CpG context) 1.2%
Percentage methylation (CHG context) 4.5%
Percentage methylation (CHH context) 1.0%

M-Bias Plot