Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-214_S30_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-214_S30_L004_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 624
Pairs without alignments under any condition 99376
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 24470
Methylated C's in CpG context 77
Methylated C's in CHG context 243
Methylated C's in CHH context 99
Methylated C's in Unknown context 2
Unmethylated C's in CpG context 6942
Unmethylated C's in CHG context 6758
Unmethylated C's in CHH context 10351
Unmethylated C's in Unknown context 9
Percentage methylation (CpG context) 1.1%
Percentage methylation (CHG context) 3.5%
Percentage methylation (CHH context) 0.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 423 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 201 original bottom strand

Deduplication

Alignments analysed 624
Unique alignments 561
Duplicates removed 63
Duplicated alignments were found at 38 different positions

Cytosine Methylation after Extraction

Total C's analysed 14784
Methylated C's in CpG context 49
Methylated C's in CHG context 143
Methylated C's in CHH context 62
Unmethylated C's in CpG context 4185
Unmethylated C's in CHG context 3998
Unmethylated C's in CHH context 6347
Percentage methylation (CpG context) 1.2%
Percentage methylation (CHG context) 3.5%
Percentage methylation (CHH context) 1.0%

M-Bias Plot