Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-209_S29_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-209_S29_L004_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 640
Pairs without alignments under any condition 99360
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 24643
Methylated C's in CpG context 93
Methylated C's in CHG context 308
Methylated C's in CHH context 71
Methylated C's in Unknown context 4
Unmethylated C's in CpG context 7101
Unmethylated C's in CHG context 6882
Unmethylated C's in CHH context 10188
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 1.3%
Percentage methylation (CHG context) 4.3%
Percentage methylation (CHH context) 0.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 456 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 184 original bottom strand

Deduplication

Alignments analysed 640
Unique alignments 590
Duplicates removed 50
Duplicated alignments were found at 43 different positions

Cytosine Methylation after Extraction

Total C's analysed 14628
Methylated C's in CpG context 55
Methylated C's in CHG context 175
Methylated C's in CHH context 46
Unmethylated C's in CpG context 4196
Unmethylated C's in CHG context 4002
Unmethylated C's in CHH context 6154
Percentage methylation (CpG context) 1.3%
Percentage methylation (CHG context) 4.2%
Percentage methylation (CHH context) 0.7%

M-Bias Plot