Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-206_S27_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-206_S27_L004_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 513
Pairs without alignments under any condition 99487
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 17988
Methylated C's in CpG context 68
Methylated C's in CHG context 201
Methylated C's in CHH context 83
Methylated C's in Unknown context 2
Unmethylated C's in CpG context 5111
Unmethylated C's in CHG context 4998
Unmethylated C's in CHH context 7527
Unmethylated C's in Unknown context 13
Percentage methylation (CpG context) 1.3%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 1.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 346 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 167 original bottom strand

Deduplication

Alignments analysed 513
Unique alignments 473
Duplicates removed 40
Duplicated alignments were found at 29 different positions

Cytosine Methylation after Extraction

Total C's analysed 10280
Methylated C's in CpG context 37
Methylated C's in CHG context 112
Methylated C's in CHH context 54
Unmethylated C's in CpG context 2932
Unmethylated C's in CHG context 2805
Unmethylated C's in CHH context 4340
Percentage methylation (CpG context) 1.2%
Percentage methylation (CHG context) 3.8%
Percentage methylation (CHH context) 1.2%

M-Bias Plot