Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-205_S26_L004_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-205_S26_L004_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 505
Pairs without alignments under any condition 99495
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 17949
Methylated C's in CpG context 78
Methylated C's in CHG context 205
Methylated C's in CHH context 78
Methylated C's in Unknown context 2
Unmethylated C's in CpG context 5146
Unmethylated C's in CHG context 4965
Unmethylated C's in CHH context 7477
Unmethylated C's in Unknown context 2
Percentage methylation (CpG context) 1.5%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 1.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 332 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 173 original bottom strand

Deduplication

Alignments analysed 505
Unique alignments 467
Duplicates removed 38
Duplicated alignments were found at 29 different positions

Cytosine Methylation after Extraction

Total C's analysed 10791
Methylated C's in CpG context 46
Methylated C's in CHG context 119
Methylated C's in CHH context 49
Unmethylated C's in CpG context 3064
Unmethylated C's in CHG context 2993
Unmethylated C's in CHH context 4520
Percentage methylation (CpG context) 1.5%
Percentage methylation (CHG context) 3.8%
Percentage methylation (CHH context) 1.1%

M-Bias Plot