Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-200_S25_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-200_S25_L003_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 472
Pairs without alignments under any condition 99528
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 16786
Methylated C's in CpG context 54
Methylated C's in CHG context 226
Methylated C's in CHH context 92
Methylated C's in Unknown context 2
Unmethylated C's in CpG context 4640
Unmethylated C's in CHG context 4649
Unmethylated C's in CHH context 7125
Unmethylated C's in Unknown context 7
Percentage methylation (CpG context) 1.2%
Percentage methylation (CHG context) 4.6%
Percentage methylation (CHH context) 1.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 322 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 150 original bottom strand

Deduplication

Alignments analysed 472
Unique alignments 430
Duplicates removed 42
Duplicated alignments were found at 34 different positions

Cytosine Methylation after Extraction

Total C's analysed 9743
Methylated C's in CpG context 31
Methylated C's in CHG context 129
Methylated C's in CHH context 52
Unmethylated C's in CpG context 2716
Unmethylated C's in CHG context 2646
Unmethylated C's in CHH context 4169
Percentage methylation (CpG context) 1.1%
Percentage methylation (CHG context) 4.6%
Percentage methylation (CHH context) 1.2%

M-Bias Plot