Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-194_S23_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-194_S23_L003_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 608
Pairs without alignments under any condition 99392
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 24315
Methylated C's in CpG context 90
Methylated C's in CHG context 279
Methylated C's in CHH context 102
Methylated C's in Unknown context 3
Unmethylated C's in CpG context 7146
Unmethylated C's in CHG context 6537
Unmethylated C's in CHH context 10161
Unmethylated C's in Unknown context 12
Percentage methylation (CpG context) 1.2%
Percentage methylation (CHG context) 4.1%
Percentage methylation (CHH context) 1.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 423 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 185 original bottom strand

Deduplication

Alignments analysed 608
Unique alignments 539
Duplicates removed 69
Duplicated alignments were found at 43 different positions

Cytosine Methylation after Extraction

Total C's analysed 14288
Methylated C's in CpG context 46
Methylated C's in CHG context 162
Methylated C's in CHH context 73
Unmethylated C's in CpG context 4116
Unmethylated C's in CHG context 3776
Unmethylated C's in CHH context 6115
Percentage methylation (CpG context) 1.1%
Percentage methylation (CHG context) 4.1%
Percentage methylation (CHH context) 1.2%

M-Bias Plot