Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-188_S21_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-188_S21_L003_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 560
Pairs without alignments under any condition 99440
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 19403
Methylated C's in CpG context 84
Methylated C's in CHG context 229
Methylated C's in CHH context 87
Methylated C's in Unknown context 13
Unmethylated C's in CpG context 5516
Unmethylated C's in CHG context 5264
Unmethylated C's in CHH context 8223
Unmethylated C's in Unknown context 8
Percentage methylation (CpG context) 1.5%
Percentage methylation (CHG context) 4.2%
Percentage methylation (CHH context) 1.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 379 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 181 original bottom strand

Deduplication

Alignments analysed 560
Unique alignments 530
Duplicates removed 30
Duplicated alignments were found at 26 different positions

Cytosine Methylation after Extraction

Total C's analysed 11593
Methylated C's in CpG context 47
Methylated C's in CHG context 132
Methylated C's in CHH context 56
Unmethylated C's in CpG context 3255
Unmethylated C's in CHG context 3131
Unmethylated C's in CHH context 4972
Percentage methylation (CpG context) 1.4%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 1.1%

M-Bias Plot