Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-185_S19_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-185_S19_L003_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 499
Pairs without alignments under any condition 99501
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 19174
Methylated C's in CpG context 89
Methylated C's in CHG context 196
Methylated C's in CHH context 77
Methylated C's in Unknown context 8
Unmethylated C's in CpG context 5395
Unmethylated C's in CHG context 5101
Unmethylated C's in CHH context 8316
Unmethylated C's in Unknown context 7
Percentage methylation (CpG context) 1.6%
Percentage methylation (CHG context) 3.7%
Percentage methylation (CHH context) 0.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 331 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 168 original bottom strand

Deduplication

Alignments analysed 499
Unique alignments 464
Duplicates removed 35
Duplicated alignments were found at 28 different positions

Cytosine Methylation after Extraction

Total C's analysed 11528
Methylated C's in CpG context 53
Methylated C's in CHG context 122
Methylated C's in CHH context 44
Unmethylated C's in CpG context 3227
Unmethylated C's in CHG context 2992
Unmethylated C's in CHH context 5090
Percentage methylation (CpG context) 1.6%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 0.9%

M-Bias Plot