Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-182_S17_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-182_S17_L003_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 466
Pairs without alignments under any condition 99534
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 19438
Methylated C's in CpG context 57
Methylated C's in CHG context 189
Methylated C's in CHH context 75
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 5560
Unmethylated C's in CHG context 5249
Unmethylated C's in CHH context 8308
Unmethylated C's in Unknown context 2
Percentage methylation (CpG context) 1.0%
Percentage methylation (CHG context) 3.5%
Percentage methylation (CHH context) 0.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 304 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 162 original bottom strand

Deduplication

Alignments analysed 466
Unique alignments 439
Duplicates removed 27
Duplicated alignments were found at 23 different positions

Cytosine Methylation after Extraction

Total C's analysed 12269
Methylated C's in CpG context 36
Methylated C's in CHG context 114
Methylated C's in CHH context 50
Unmethylated C's in CpG context 3461
Unmethylated C's in CHG context 3259
Unmethylated C's in CHH context 5349
Percentage methylation (CpG context) 1.0%
Percentage methylation (CHG context) 3.4%
Percentage methylation (CHH context) 0.9%

M-Bias Plot