Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-181_S16_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-181_S16_L003_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 420
Pairs without alignments under any condition 99580
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 17504
Methylated C's in CpG context 57
Methylated C's in CHG context 146
Methylated C's in CHH context 66
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 4851
Unmethylated C's in CHG context 4794
Unmethylated C's in CHH context 7590
Unmethylated C's in Unknown context 5
Percentage methylation (CpG context) 1.2%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 0.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 270 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 150 original bottom strand

Deduplication

Alignments analysed 420
Unique alignments 404
Duplicates removed 16
Duplicated alignments were found at 14 different positions

Cytosine Methylation after Extraction

Total C's analysed 11808
Methylated C's in CpG context 38
Methylated C's in CHG context 100
Methylated C's in CHH context 51
Unmethylated C's in CpG context 3223
Unmethylated C's in CHG context 3187
Unmethylated C's in CHH context 5209
Percentage methylation (CpG context) 1.2%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 1.0%

M-Bias Plot