Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-176_S15_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-176_S15_L003_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 1154
Pairs without alignments under any condition 98846
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 47248
Methylated C's in CpG context 158
Methylated C's in CHG context 516
Methylated C's in CHH context 187
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 13283
Unmethylated C's in CHG context 12806
Unmethylated C's in CHH context 20298
Unmethylated C's in Unknown context 24
Percentage methylation (CpG context) 1.2%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 0.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 765 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 389 original bottom strand

Deduplication

Alignments analysed 1154
Unique alignments 1024
Duplicates removed 130
Duplicated alignments were found at 81 different positions

Cytosine Methylation after Extraction

Total C's analysed 28773
Methylated C's in CpG context 89
Methylated C's in CHG context 313
Methylated C's in CHH context 116
Unmethylated C's in CpG context 8039
Unmethylated C's in CHG context 7716
Unmethylated C's in CHH context 12500
Percentage methylation (CpG context) 1.1%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 0.9%

M-Bias Plot