Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-175_S14_L003_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-175_S14_L003_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 792
Pairs without alignments under any condition 99208
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 32529
Methylated C's in CpG context 85
Methylated C's in CHG context 351
Methylated C's in CHH context 128
Methylated C's in Unknown context 4
Unmethylated C's in CpG context 9343
Unmethylated C's in CHG context 8875
Unmethylated C's in CHH context 13747
Unmethylated C's in Unknown context 5
Percentage methylation (CpG context) 0.9%
Percentage methylation (CHG context) 3.8%
Percentage methylation (CHH context) 0.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 543 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 249 original bottom strand

Deduplication

Alignments analysed 792
Unique alignments 701
Duplicates removed 91
Duplicated alignments were found at 61 different positions

Cytosine Methylation after Extraction

Total C's analysed 19358
Methylated C's in CpG context 55
Methylated C's in CHG context 197
Methylated C's in CHH context 79
Unmethylated C's in CpG context 5529
Unmethylated C's in CHG context 5183
Unmethylated C's in CHH context 8315
Percentage methylation (CpG context) 1.0%
Percentage methylation (CHG context) 3.7%
Percentage methylation (CHH context) 0.9%

M-Bias Plot