Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-169_S12_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-169_S12_L002_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 322
Pairs without alignments under any condition 99678
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 12209
Methylated C's in CpG context 55
Methylated C's in CHG context 153
Methylated C's in CHH context 62
Methylated C's in Unknown context 2
Unmethylated C's in CpG context 3518
Unmethylated C's in CHG context 3274
Unmethylated C's in CHH context 5147
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 1.5%
Percentage methylation (CHG context) 4.5%
Percentage methylation (CHH context) 1.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 220 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 102 original bottom strand

Deduplication

Alignments analysed 322
Unique alignments 306
Duplicates removed 16
Duplicated alignments were found at 12 different positions

Cytosine Methylation after Extraction

Total C's analysed 7462
Methylated C's in CpG context 35
Methylated C's in CHG context 97
Methylated C's in CHH context 37
Unmethylated C's in CpG context 2155
Unmethylated C's in CHG context 1968
Unmethylated C's in CHH context 3170
Percentage methylation (CpG context) 1.6%
Percentage methylation (CHG context) 4.7%
Percentage methylation (CHH context) 1.2%

M-Bias Plot