Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-167_S10_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-167_S10_L002_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 781
Pairs without alignments under any condition 99219
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 36053
Methylated C's in CpG context 189
Methylated C's in CHG context 367
Methylated C's in CHH context 300
Methylated C's in Unknown context 5
Unmethylated C's in CpG context 10550
Unmethylated C's in CHG context 10313
Unmethylated C's in CHH context 14334
Unmethylated C's in Unknown context 13
Percentage methylation (CpG context) 1.8%
Percentage methylation (CHG context) 3.4%
Percentage methylation (CHH context) 2.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 497 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 284 original bottom strand

Deduplication

Alignments analysed 781
Unique alignments 778
Duplicates removed 3
Duplicated alignments were found at 3 different positions

Cytosine Methylation after Extraction

Total C's analysed 25908
Methylated C's in CpG context 141
Methylated C's in CHG context 274
Methylated C's in CHH context 229
Unmethylated C's in CpG context 7575
Unmethylated C's in CHG context 7400
Unmethylated C's in CHH context 10289
Percentage methylation (CpG context) 1.8%
Percentage methylation (CHG context) 3.6%
Percentage methylation (CHH context) 2.2%

M-Bias Plot