Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-161_S8_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-161_S8_L002_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 587
Pairs without alignments under any condition 99413
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 22950
Methylated C's in CpG context 98
Methylated C's in CHG context 237
Methylated C's in CHH context 100
Methylated C's in Unknown context 5
Unmethylated C's in CpG context 6548
Unmethylated C's in CHG context 6286
Unmethylated C's in CHH context 9681
Unmethylated C's in Unknown context 4
Percentage methylation (CpG context) 1.5%
Percentage methylation (CHG context) 3.6%
Percentage methylation (CHH context) 1.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 370 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 217 original bottom strand

Deduplication

Alignments analysed 587
Unique alignments 533
Duplicates removed 54
Duplicated alignments were found at 38 different positions

Cytosine Methylation after Extraction

Total C's analysed 13454
Methylated C's in CpG context 56
Methylated C's in CHG context 130
Methylated C's in CHH context 61
Unmethylated C's in CpG context 3815
Unmethylated C's in CHG context 3620
Unmethylated C's in CHH context 5772
Percentage methylation (CpG context) 1.4%
Percentage methylation (CHG context) 3.5%
Percentage methylation (CHH context) 1.0%

M-Bias Plot