Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-160_S7_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-160_S7_L002_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 1825
Pairs without alignments under any condition 98175
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 67790
Methylated C's in CpG context 214
Methylated C's in CHG context 788
Methylated C's in CHH context 347
Methylated C's in Unknown context 8
Unmethylated C's in CpG context 19193
Unmethylated C's in CHG context 18392
Unmethylated C's in CHH context 28856
Unmethylated C's in Unknown context 24
Percentage methylation (CpG context) 1.1%
Percentage methylation (CHG context) 4.1%
Percentage methylation (CHH context) 1.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 1215 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 610 original bottom strand

Deduplication

Alignments analysed 1825
Unique alignments 1540
Duplicates removed 285
Duplicated alignments were found at 159 different positions

Cytosine Methylation after Extraction

Total C's analysed 37041
Methylated C's in CpG context 119
Methylated C's in CHG context 404
Methylated C's in CHH context 194
Unmethylated C's in CpG context 10386
Unmethylated C's in CHG context 9934
Unmethylated C's in CHH context 16004
Percentage methylation (CpG context) 1.1%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 1.2%

M-Bias Plot