Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-159_S6_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-159_S6_L002_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 488
Pairs without alignments under any condition 99512
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 19125
Methylated C's in CpG context 72
Methylated C's in CHG context 192
Methylated C's in CHH context 85
Methylated C's in Unknown context 2
Unmethylated C's in CpG context 5542
Unmethylated C's in CHG context 5229
Unmethylated C's in CHH context 8005
Unmethylated C's in Unknown context 4
Percentage methylation (CpG context) 1.3%
Percentage methylation (CHG context) 3.5%
Percentage methylation (CHH context) 1.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 350 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 138 original bottom strand

Deduplication

Alignments analysed 488
Unique alignments 452
Duplicates removed 36
Duplicated alignments were found at 29 different positions

Cytosine Methylation after Extraction

Total C's analysed 12096
Methylated C's in CpG context 42
Methylated C's in CHG context 122
Methylated C's in CHH context 55
Unmethylated C's in CpG context 3449
Unmethylated C's in CHG context 3269
Unmethylated C's in CHH context 5159
Percentage methylation (CpG context) 1.2%
Percentage methylation (CHG context) 3.6%
Percentage methylation (CHH context) 1.1%

M-Bias Plot