Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-151_S2_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-151_S2_L002_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 621
Pairs without alignments under any condition 99379
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 24525
Methylated C's in CpG context 75
Methylated C's in CHG context 309
Methylated C's in CHH context 104
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 6962
Unmethylated C's in CHG context 6893
Unmethylated C's in CHH context 10182
Unmethylated C's in Unknown context 2
Percentage methylation (CpG context) 1.1%
Percentage methylation (CHG context) 4.3%
Percentage methylation (CHH context) 1.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 421 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 200 original bottom strand

Deduplication

Alignments analysed 621
Unique alignments 554
Duplicates removed 67
Duplicated alignments were found at 42 different positions

Cytosine Methylation after Extraction

Total C's analysed 14731
Methylated C's in CpG context 52
Methylated C's in CHG context 173
Methylated C's in CHH context 63
Unmethylated C's in CpG context 4112
Unmethylated C's in CHG context 4085
Unmethylated C's in CHH context 6246
Percentage methylation (CpG context) 1.2%
Percentage methylation (CHG context) 4.1%
Percentage methylation (CHH context) 1.0%

M-Bias Plot