Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-145_S38_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-145_S38_L005_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 461
Pairs without alignments under any condition 99539
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 19052
Methylated C's in CpG context 66
Methylated C's in CHG context 200
Methylated C's in CHH context 68
Methylated C's in Unknown context 4
Unmethylated C's in CpG context 5564
Unmethylated C's in CHG context 5129
Unmethylated C's in CHH context 8025
Unmethylated C's in Unknown context 9
Percentage methylation (CpG context) 1.2%
Percentage methylation (CHG context) 3.8%
Percentage methylation (CHH context) 0.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 303 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 158 original bottom strand

Deduplication

Alignments analysed 461
Unique alignments 438
Duplicates removed 23
Duplicated alignments were found at 17 different positions

Cytosine Methylation after Extraction

Total C's analysed 12533
Methylated C's in CpG context 38
Methylated C's in CHG context 131
Methylated C's in CHH context 48
Unmethylated C's in CpG context 3610
Unmethylated C's in CHG context 3351
Unmethylated C's in CHH context 5355
Percentage methylation (CpG context) 1.0%
Percentage methylation (CHG context) 3.8%
Percentage methylation (CHH context) 0.9%

M-Bias Plot