Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-135_S35_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-135_S35_L005_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 402
Pairs without alignments under any condition 99598
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 15423
Methylated C's in CpG context 51
Methylated C's in CHG context 204
Methylated C's in CHH context 41
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 4476
Unmethylated C's in CHG context 4350
Unmethylated C's in CHH context 6301
Unmethylated C's in Unknown context 4
Percentage methylation (CpG context) 1.1%
Percentage methylation (CHG context) 4.5%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 264 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 138 original bottom strand

Deduplication

Alignments analysed 402
Unique alignments 382
Duplicates removed 20
Duplicated alignments were found at 16 different positions

Cytosine Methylation after Extraction

Total C's analysed 9552
Methylated C's in CpG context 33
Methylated C's in CHG context 113
Methylated C's in CHH context 23
Unmethylated C's in CpG context 2762
Unmethylated C's in CHG context 2680
Unmethylated C's in CHH context 3941
Percentage methylation (CpG context) 1.2%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 0.6%

M-Bias Plot