Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-127_S33_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-127_S33_L005_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 472
Pairs without alignments under any condition 99528
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 19671
Methylated C's in CpG context 43
Methylated C's in CHG context 178
Methylated C's in CHH context 51
Methylated C's in Unknown context 2
Unmethylated C's in CpG context 5723
Unmethylated C's in CHG context 5518
Unmethylated C's in CHH context 8158
Unmethylated C's in Unknown context 2
Percentage methylation (CpG context) 0.7%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 315 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 157 original bottom strand

Deduplication

Alignments analysed 472
Unique alignments 454
Duplicates removed 18
Duplicated alignments were found at 13 different positions

Cytosine Methylation after Extraction

Total C's analysed 13318
Methylated C's in CpG context 35
Methylated C's in CHG context 121
Methylated C's in CHH context 37
Unmethylated C's in CpG context 3839
Unmethylated C's in CHG context 3702
Unmethylated C's in CHH context 5584
Percentage methylation (CpG context) 0.9%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 0.7%

M-Bias Plot