Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-120_S32_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-120_S32_L005_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 509
Pairs without alignments under any condition 99491
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 20623
Methylated C's in CpG context 66
Methylated C's in CHG context 185
Methylated C's in CHH context 72
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 5916
Unmethylated C's in CHG context 5838
Unmethylated C's in CHH context 8546
Unmethylated C's in Unknown context 4
Percentage methylation (CpG context) 1.1%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 0.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 338 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 171 original bottom strand

Deduplication

Alignments analysed 509
Unique alignments 485
Duplicates removed 24
Duplicated alignments were found at 17 different positions

Cytosine Methylation after Extraction

Total C's analysed 13549
Methylated C's in CpG context 42
Methylated C's in CHG context 117
Methylated C's in CHH context 51
Unmethylated C's in CpG context 3848
Unmethylated C's in CHG context 3831
Unmethylated C's in CHH context 5660
Percentage methylation (CpG context) 1.1%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 0.9%

M-Bias Plot