Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-119_S31_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-119_S31_L005_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 1825
Pairs without alignments under any condition 98175
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 78115
Methylated C's in CpG context 207
Methylated C's in CHG context 655
Methylated C's in CHH context 232
Methylated C's in Unknown context 2
Unmethylated C's in CpG context 22679
Unmethylated C's in CHG context 22265
Unmethylated C's in CHH context 32077
Unmethylated C's in Unknown context 30
Percentage methylation (CpG context) 0.9%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 0.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 1217 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 608 original bottom strand

Deduplication

Alignments analysed 1825
Unique alignments 1741
Duplicates removed 84
Duplicated alignments were found at 67 different positions

Cytosine Methylation after Extraction

Total C's analysed 53002
Methylated C's in CpG context 144
Methylated C's in CHG context 442
Methylated C's in CHH context 176
Unmethylated C's in CpG context 15363
Unmethylated C's in CHG context 14896
Unmethylated C's in CHH context 21981
Percentage methylation (CpG context) 0.9%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 0.8%

M-Bias Plot