Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-113_S30_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-113_S30_L005_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 737
Pairs without alignments under any condition 99263
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 29996
Methylated C's in CpG context 81
Methylated C's in CHG context 261
Methylated C's in CHH context 100
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 8622
Unmethylated C's in CHG context 8210
Unmethylated C's in CHH context 12722
Unmethylated C's in Unknown context 3
Percentage methylation (CpG context) 0.9%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 0.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 487 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 250 original bottom strand

Deduplication

Alignments analysed 737
Unique alignments 693
Duplicates removed 44
Duplicated alignments were found at 33 different positions

Cytosine Methylation after Extraction

Total C's analysed 19356
Methylated C's in CpG context 50
Methylated C's in CHG context 169
Methylated C's in CHH context 71
Unmethylated C's in CpG context 5494
Unmethylated C's in CHG context 5243
Unmethylated C's in CHH context 8329
Percentage methylation (CpG context) 0.9%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 0.8%

M-Bias Plot