Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-111_S29_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-111_S29_L005_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 340
Pairs without alignments under any condition 99660
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 13846
Methylated C's in CpG context 37
Methylated C's in CHG context 114
Methylated C's in CHH context 34
Methylated C's in Unknown context 2
Unmethylated C's in CpG context 3990
Unmethylated C's in CHG context 3772
Unmethylated C's in CHH context 5899
Unmethylated C's in Unknown context 1
Percentage methylation (CpG context) 0.9%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 0.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 220 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 120 original bottom strand

Deduplication

Alignments analysed 340
Unique alignments 324
Duplicates removed 16
Duplicated alignments were found at 11 different positions

Cytosine Methylation after Extraction

Total C's analysed 8929
Methylated C's in CpG context 25
Methylated C's in CHG context 77
Methylated C's in CHH context 24
Unmethylated C's in CpG context 2521
Unmethylated C's in CHG context 2422
Unmethylated C's in CHH context 3860
Percentage methylation (CpG context) 1.0%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 0.6%

M-Bias Plot