Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-104_S28_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-104_S28_L005_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 512
Pairs without alignments under any condition 99488
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 21465
Methylated C's in CpG context 67
Methylated C's in CHG context 184
Methylated C's in CHH context 76
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 6109
Unmethylated C's in CHG context 5946
Unmethylated C's in CHH context 9083
Unmethylated C's in Unknown context 6
Percentage methylation (CpG context) 1.1%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 0.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 341 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 171 original bottom strand

Deduplication

Alignments analysed 512
Unique alignments 497
Duplicates removed 15
Duplicated alignments were found at 12 different positions

Cytosine Methylation after Extraction

Total C's analysed 14389
Methylated C's in CpG context 50
Methylated C's in CHG context 129
Methylated C's in CHH context 54
Unmethylated C's in CpG context 4077
Unmethylated C's in CHG context 3941
Unmethylated C's in CHH context 6138
Percentage methylation (CpG context) 1.2%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 0.9%

M-Bias Plot