Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-103_S27_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-103_S27_L005_R2_001_val_2.fq.gz

Data processed at 14:11 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 626
Pairs without alignments under any condition 99374
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 27210
Methylated C's in CpG context 69
Methylated C's in CHG context 210
Methylated C's in CHH context 82
Methylated C's in Unknown context 2
Unmethylated C's in CpG context 7706
Unmethylated C's in CHG context 7606
Unmethylated C's in CHH context 11537
Unmethylated C's in Unknown context 6
Percentage methylation (CpG context) 0.9%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 0.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 402 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 224 original bottom strand

Deduplication

Alignments analysed 626
Unique alignments 611
Duplicates removed 15
Duplicated alignments were found at 11 different positions

Cytosine Methylation after Extraction

Total C's analysed 18654
Methylated C's in CpG context 49
Methylated C's in CHG context 153
Methylated C's in CHH context 58
Unmethylated C's in CpG context 5272
Unmethylated C's in CHG context 5187
Unmethylated C's in CHH context 7935
Percentage methylation (CpG context) 0.9%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 0.7%

M-Bias Plot