Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-170_S13_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-170_S13_L002_R2_001_val_2.fq.gz

Data processed at 13:25 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 464
Pairs without alignments under any condition 99536
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 16764
Methylated C's in CpG context 51
Methylated C's in CHG context 230
Methylated C's in CHH context 80
Methylated C's in Unknown context 2
Unmethylated C's in CpG context 4862
Unmethylated C's in CHG context 4524
Unmethylated C's in CHH context 7017
Unmethylated C's in Unknown context 4
Percentage methylation (CpG context) 1.0%
Percentage methylation (CHG context) 4.8%
Percentage methylation (CHH context) 1.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 313 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 151 original bottom strand

Deduplication

Alignments analysed 464
Unique alignments 446
Duplicates removed 18
Duplicated alignments were found at 15 different positions

Cytosine Methylation after Extraction

Total C's analysed 10292
Methylated C's in CpG context 33
Methylated C's in CHG context 134
Methylated C's in CHH context 51
Unmethylated C's in CpG context 2966
Unmethylated C's in CHG context 2731
Unmethylated C's in CHH context 4377
Percentage methylation (CpG context) 1.1%
Percentage methylation (CHG context) 4.7%
Percentage methylation (CHH context) 1.2%

M-Bias Plot