Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-168_S11_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-168_S11_L002_R2_001_val_2.fq.gz

Data processed at 13:25 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 579
Pairs without alignments under any condition 99421
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 23642
Methylated C's in CpG context 71
Methylated C's in CHG context 251
Methylated C's in CHH context 126
Methylated C's in Unknown context 2
Unmethylated C's in CpG context 6804
Unmethylated C's in CHG context 6632
Unmethylated C's in CHH context 9758
Unmethylated C's in Unknown context 7
Percentage methylation (CpG context) 1.0%
Percentage methylation (CHG context) 3.6%
Percentage methylation (CHH context) 1.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 397 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 182 original bottom strand

Deduplication

Alignments analysed 579
Unique alignments 545
Duplicates removed 34
Duplicated alignments were found at 27 different positions

Cytosine Methylation after Extraction

Total C's analysed 15205
Methylated C's in CpG context 50
Methylated C's in CHG context 157
Methylated C's in CHH context 81
Unmethylated C's in CpG context 4307
Unmethylated C's in CHG context 4240
Unmethylated C's in CHH context 6370
Percentage methylation (CpG context) 1.1%
Percentage methylation (CHG context) 3.6%
Percentage methylation (CHH context) 1.3%

M-Bias Plot