Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-162_S9_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-162_S9_L002_R2_001_val_2.fq.gz

Data processed at 13:25 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 615
Pairs without alignments under any condition 99385
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 22576
Methylated C's in CpG context 83
Methylated C's in CHG context 302
Methylated C's in CHH context 127
Methylated C's in Unknown context 4
Unmethylated C's in CpG context 6495
Unmethylated C's in CHG context 6133
Unmethylated C's in CHH context 9436
Unmethylated C's in Unknown context 6
Percentage methylation (CpG context) 1.3%
Percentage methylation (CHG context) 4.7%
Percentage methylation (CHH context) 1.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 419 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 196 original bottom strand

Deduplication

Alignments analysed 615
Unique alignments 577
Duplicates removed 38
Duplicated alignments were found at 31 different positions

Cytosine Methylation after Extraction

Total C's analysed 13600
Methylated C's in CpG context 46
Methylated C's in CHG context 163
Methylated C's in CHH context 77
Unmethylated C's in CpG context 3867
Unmethylated C's in CHG context 3658
Unmethylated C's in CHH context 5789
Percentage methylation (CpG context) 1.2%
Percentage methylation (CHG context) 4.3%
Percentage methylation (CHH context) 1.3%

M-Bias Plot