Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-154_S5_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-154_S5_L002_R2_001_val_2.fq.gz

Data processed at 13:25 on 2019-11-04


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 829
Pairs without alignments under any condition 99171
Pairs that did not map uniquely 0
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 33555
Methylated C's in CpG context 112
Methylated C's in CHG context 380
Methylated C's in CHH context 145
Methylated C's in Unknown context 5
Unmethylated C's in CpG context 9663
Unmethylated C's in CHG context 9316
Unmethylated C's in CHH context 13939
Unmethylated C's in Unknown context 15
Percentage methylation (CpG context) 1.1%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 1.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 565 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 264 original bottom strand

Deduplication

Alignments analysed 829
Unique alignments 734
Duplicates removed 95
Duplicated alignments were found at 55 different positions

Cytosine Methylation after Extraction

Total C's analysed 19966
Methylated C's in CpG context 72
Methylated C's in CHG context 222
Methylated C's in CHH context 91
Unmethylated C's in CpG context 5701
Unmethylated C's in CHG context 5462
Unmethylated C's in CHH context 8418
Percentage methylation (CpG context) 1.2%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 1.1%

M-Bias Plot