Bismark

Bismark Processing Report

/gscratch/scrubbed/sr320/0924/EPI-170_S13_L002_R1_001_val_1.20bp-trim.fq.gz and /gscratch/scrubbed/sr320/0924/EPI-170_S13_L002_R2_001_val_2.20bp-trim.fq.gz

Data processed at 07:08 on 2019-09-26


Alignment Stats

Sequence pairs analysed in total 27965199
Paired-end alignments with a unique best hit 9838839
Pairs without alignments under any condition 15019108
Pairs that did not map uniquely 3107252
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 225533821
Methylated C's in CpG context 7206109
Methylated C's in CHG context 672061
Methylated C's in CHH context 2203353
Methylated C's in Unknown context 24430
Unmethylated C's in CpG context 23463775
Unmethylated C's in CHG context 46888524
Unmethylated C's in CHH context 145099999
Unmethylated C's in Unknown context 469468
Percentage methylation (CpG context) 23.5%
Percentage methylation (CHG context) 1.4%
Percentage methylation (CHH context) 1.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4818414 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5020425 original bottom strand

Deduplication

Alignments analysed 9838839
Unique alignments 7528619
Duplicates removed 2310220
Duplicated alignments were found at 1334576 different positions

Cytosine Methylation after Extraction

Total C's analysed 116035249
Methylated C's in CpG context 3802971
Methylated C's in CHG context 286488
Methylated C's in CHH context 1168470
Unmethylated C's in CpG context 10991404
Unmethylated C's in CHG context 23293231
Unmethylated C's in CHH context 76492685
Percentage methylation (CpG context) 25.7%
Percentage methylation (CHG context) 1.2%
Percentage methylation (CHH context) 1.5%

M-Bias Plot