Bismark

Bismark Processing Report

/gscratch/scrubbed/sr320/0924/EPI-169_S12_L002_R1_001_val_1.20bp-trim.fq.gz and /gscratch/scrubbed/sr320/0924/EPI-169_S12_L002_R2_001_val_2.20bp-trim.fq.gz

Data processed at 07:08 on 2019-09-26


Alignment Stats

Sequence pairs analysed in total 21368558
Paired-end alignments with a unique best hit 8099481
Pairs without alignments under any condition 10769512
Pairs that did not map uniquely 2499565
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 188365927
Methylated C's in CpG context 6511388
Methylated C's in CHG context 609098
Methylated C's in CHH context 2844774
Methylated C's in Unknown context 30187
Unmethylated C's in CpG context 19075660
Unmethylated C's in CHG context 39689208
Unmethylated C's in CHH context 119635799
Unmethylated C's in Unknown context 386938
Percentage methylation (CpG context) 25.4%
Percentage methylation (CHG context) 1.5%
Percentage methylation (CHH context) 2.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3966747 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4132734 original bottom strand

Deduplication

Alignments analysed 8099481
Unique alignments 6203096
Duplicates removed 1896385
Duplicated alignments were found at 1133854 different positions

Cytosine Methylation after Extraction

Total C's analysed 97770671
Methylated C's in CpG context 3441248
Methylated C's in CHG context 267831
Methylated C's in CHH context 1560958
Unmethylated C's in CpG context 9032975
Unmethylated C's in CHG context 19939768
Unmethylated C's in CHH context 63527891
Percentage methylation (CpG context) 27.6%
Percentage methylation (CHG context) 1.3%
Percentage methylation (CHH context) 2.4%

M-Bias Plot