Bismark

Bismark Processing Report

/gscratch/scrubbed/sr320/0924/EPI-168_S11_L002_R1_001_val_1.20bp-trim.fq.gz and /gscratch/scrubbed/sr320/0924/EPI-168_S11_L002_R2_001_val_2.20bp-trim.fq.gz

Data processed at 07:08 on 2019-09-26


Alignment Stats

Sequence pairs analysed in total 22681213
Paired-end alignments with a unique best hit 8839686
Pairs without alignments under any condition 11631582
Pairs that did not map uniquely 2209945
Genomic sequence context not extractable (edges of chromosomes) 2

Cytosine Methylation

Total C's analysed 219313655
Methylated C's in CpG context 7328516
Methylated C's in CHG context 595495
Methylated C's in CHH context 2626182
Methylated C's in Unknown context 26743
Unmethylated C's in CpG context 22293832
Unmethylated C's in CHG context 46305078
Unmethylated C's in CHH context 140164552
Unmethylated C's in Unknown context 468533
Percentage methylation (CpG context) 24.7%
Percentage methylation (CHG context) 1.3%
Percentage methylation (CHH context) 1.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4322133 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4517551 original bottom strand

Deduplication

Alignments analysed 8839684
Unique alignments 6388240
Duplicates removed 2451444
Duplicated alignments were found at 1460264 different positions

Cytosine Methylation after Extraction

Total C's analysed 108420009
Methylated C's in CpG context 3766086
Methylated C's in CHG context 261539
Methylated C's in CHH context 1403272
Unmethylated C's in CpG context 9999893
Unmethylated C's in CHG context 22034468
Unmethylated C's in CHH context 70954751
Percentage methylation (CpG context) 27.4%
Percentage methylation (CHG context) 1.2%
Percentage methylation (CHH context) 1.9%

M-Bias Plot