Bismark report for: /gscratch/scrubbed/sr320/0924/EPI-162_S9_L002_R1_001_val_1.20bp-trim.fq.gz and /gscratch/scrubbed/sr320/0924/EPI-162_S9_L002_R2_001_val_2.20bp-trim.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/geoduck/v074/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 22746423 Number of paired-end alignments with a unique best hit: 7982139 Mapping efficiency: 35.1% Sequence pairs with no alignments under any condition: 12293756 Sequence pairs did not map uniquely: 2470528 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3917194 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 4064945 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 182398480 Total methylated C's in CpG context: 5917870 Total methylated C's in CHG context: 561612 Total methylated C's in CHH context: 2425318 Total methylated C's in Unknown context: 26840 Total unmethylated C's in CpG context: 18231986 Total unmethylated C's in CHG context: 37832540 Total unmethylated C's in CHH context: 117429154 Total unmethylated C's in Unknown context: 378028 C methylated in CpG context: 24.5% C methylated in CHG context: 1.5% C methylated in CHH context: 2.0% C methylated in unknown context (CN or CHN): 6.6% Bismark completed in 0d 1h 15m 19s