Bismark

Bismark Processing Report

/gscratch/scrubbed/sr320/0924/EPI-162_S9_L002_R1_001_val_1.20bp-trim.fq.gz and /gscratch/scrubbed/sr320/0924/EPI-162_S9_L002_R2_001_val_2.20bp-trim.fq.gz

Data processed at 07:08 on 2019-09-26


Alignment Stats

Sequence pairs analysed in total 22746423
Paired-end alignments with a unique best hit 7982139
Pairs without alignments under any condition 12293756
Pairs that did not map uniquely 2470528
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 182398480
Methylated C's in CpG context 5917870
Methylated C's in CHG context 561612
Methylated C's in CHH context 2425318
Methylated C's in Unknown context 26840
Unmethylated C's in CpG context 18231986
Unmethylated C's in CHG context 37832540
Unmethylated C's in CHH context 117429154
Unmethylated C's in Unknown context 378028
Percentage methylation (CpG context) 24.5%
Percentage methylation (CHG context) 1.5%
Percentage methylation (CHH context) 2.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3917194 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4064945 original bottom strand

Deduplication

Alignments analysed 7982139
Unique alignments 6171703
Duplicates removed 1810436
Duplicated alignments were found at 1100252 different positions

Cytosine Methylation after Extraction

Total C's analysed 94008308
Methylated C's in CpG context 3099669
Methylated C's in CHG context 247160
Methylated C's in CHH context 1320001
Unmethylated C's in CpG context 8848327
Unmethylated C's in CHG context 18885370
Unmethylated C's in CHH context 61607781
Percentage methylation (CpG context) 25.9%
Percentage methylation (CHG context) 1.3%
Percentage methylation (CHH context) 2.1%

M-Bias Plot