Bismark report for: /gscratch/scrubbed/sr320/0924/EPI-161_S8_L002_R1_001_val_1.20bp-trim.fq.gz and /gscratch/scrubbed/sr320/0924/EPI-161_S8_L002_R2_001_val_2.20bp-trim.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/geoduck/v074/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 24492789 Number of paired-end alignments with a unique best hit: 8347099 Mapping efficiency: 34.1% Sequence pairs with no alignments under any condition: 13726258 Sequence pairs did not map uniquely: 2419432 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 4106177 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 4240922 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 193695409 Total methylated C's in CpG context: 6288786 Total methylated C's in CHG context: 569736 Total methylated C's in CHH context: 2419489 Total methylated C's in Unknown context: 28456 Total unmethylated C's in CpG context: 19259401 Total unmethylated C's in CHG context: 39712890 Total unmethylated C's in CHH context: 125445107 Total unmethylated C's in Unknown context: 389018 C methylated in CpG context: 24.6% C methylated in CHG context: 1.4% C methylated in CHH context: 1.9% C methylated in unknown context (CN or CHN): 6.8% Bismark completed in 0d 1h 17m 30s