Bismark

Bismark Processing Report

/gscratch/scrubbed/sr320/0924/EPI-161_S8_L002_R1_001_val_1.20bp-trim.fq.gz and /gscratch/scrubbed/sr320/0924/EPI-161_S8_L002_R2_001_val_2.20bp-trim.fq.gz

Data processed at 07:08 on 2019-09-26


Alignment Stats

Sequence pairs analysed in total 24492789
Paired-end alignments with a unique best hit 8347099
Pairs without alignments under any condition 13726258
Pairs that did not map uniquely 2419432
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 193695409
Methylated C's in CpG context 6288786
Methylated C's in CHG context 569736
Methylated C's in CHH context 2419489
Methylated C's in Unknown context 28456
Unmethylated C's in CpG context 19259401
Unmethylated C's in CHG context 39712890
Unmethylated C's in CHH context 125445107
Unmethylated C's in Unknown context 389018
Percentage methylation (CpG context) 24.6%
Percentage methylation (CHG context) 1.4%
Percentage methylation (CHH context) 1.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4106177 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4240922 original bottom strand

Deduplication

Alignments analysed 8347099
Unique alignments 6156942
Duplicates removed 2190157
Duplicated alignments were found at 1384516 different positions

Cytosine Methylation after Extraction

Total C's analysed 95509068
Methylated C's in CpG context 3144991
Methylated C's in CHG context 235785
Methylated C's in CHH context 1268797
Unmethylated C's in CpG context 8852963
Unmethylated C's in CHG context 19018059
Unmethylated C's in CHH context 62988473
Percentage methylation (CpG context) 26.2%
Percentage methylation (CHG context) 1.2%
Percentage methylation (CHH context) 2.0%

M-Bias Plot