Bismark

Bismark Processing Report

/gscratch/scrubbed/sr320/0924/EPI-159_S6_L002_R1_001_val_1.20bp-trim.fq.gz and /gscratch/scrubbed/sr320/0924/EPI-159_S6_L002_R2_001_val_2.20bp-trim.fq.gz

Data processed at 07:08 on 2019-09-26


Alignment Stats

Sequence pairs analysed in total 15443938
Paired-end alignments with a unique best hit 6261661
Pairs without alignments under any condition 7560902
Pairs that did not map uniquely 1621375
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 153549770
Methylated C's in CpG context 5315174
Methylated C's in CHG context 468224
Methylated C's in CHH context 2827416
Methylated C's in Unknown context 27185
Unmethylated C's in CpG context 14496058
Unmethylated C's in CHG context 31220586
Unmethylated C's in CHH context 99222312
Unmethylated C's in Unknown context 319739
Percentage methylation (CpG context) 26.8%
Percentage methylation (CHG context) 1.5%
Percentage methylation (CHH context) 2.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3093116 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 3168545 original bottom strand

Deduplication

Alignments analysed 6261661
Unique alignments 4557124
Duplicates removed 1704537
Duplicated alignments were found at 1068007 different positions

Cytosine Methylation after Extraction

Total C's analysed 77011929
Methylated C's in CpG context 2724982
Methylated C's in CHG context 200177
Methylated C's in CHH context 1556131
Unmethylated C's in CpG context 6770348
Unmethylated C's in CHG context 15236636
Unmethylated C's in CHH context 50523655
Percentage methylation (CpG context) 28.7%
Percentage methylation (CHG context) 1.3%
Percentage methylation (CHH context) 3.0%

M-Bias Plot