Bismark report for: /gscratch/scrubbed/sr320/0924/EPI-152_S3_L002_R1_001_val_1.20bp-trim.fq.gz and /gscratch/scrubbed/sr320/0924/EPI-152_S3_L002_R2_001_val_2.20bp-trim.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/geoduck/v074/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 29841619 Number of paired-end alignments with a unique best hit: 11530248 Mapping efficiency: 38.6% Sequence pairs with no alignments under any condition: 15423787 Sequence pairs did not map uniquely: 2887584 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 5701659 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 5828588 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 281731297 Total methylated C's in CpG context: 9936392 Total methylated C's in CHG context: 780374 Total methylated C's in CHH context: 2830980 Total methylated C's in Unknown context: 32415 Total unmethylated C's in CpG context: 26480973 Total unmethylated C's in CHG context: 57771725 Total unmethylated C's in CHH context: 183930853 Total unmethylated C's in Unknown context: 575301 C methylated in CpG context: 27.3% C methylated in CHG context: 1.3% C methylated in CHH context: 1.5% C methylated in unknown context (CN or CHN): 5.3% Bismark completed in 0d 1h 44m 14s