Bismark

Bismark Processing Report

/gscratch/scrubbed/sr320/0924/EPI-152_S3_L002_R1_001_val_1.20bp-trim.fq.gz and /gscratch/scrubbed/sr320/0924/EPI-152_S3_L002_R2_001_val_2.20bp-trim.fq.gz

Data processed at 07:08 on 2019-09-26


Alignment Stats

Sequence pairs analysed in total 29841619
Paired-end alignments with a unique best hit 11530248
Pairs without alignments under any condition 15423787
Pairs that did not map uniquely 2887584
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 281731297
Methylated C's in CpG context 9936392
Methylated C's in CHG context 780374
Methylated C's in CHH context 2830980
Methylated C's in Unknown context 32415
Unmethylated C's in CpG context 26480973
Unmethylated C's in CHG context 57771725
Unmethylated C's in CHH context 183930853
Unmethylated C's in Unknown context 575301
Percentage methylation (CpG context) 27.3%
Percentage methylation (CHG context) 1.3%
Percentage methylation (CHH context) 1.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5701659 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5828588 original bottom strand

Deduplication

Alignments analysed 11530247
Unique alignments 8278241
Duplicates removed 3252006
Duplicated alignments were found at 1988314 different positions

Cytosine Methylation after Extraction

Total C's analysed 140825920
Methylated C's in CpG context 5081303
Methylated C's in CHG context 323485
Methylated C's in CHH context 1461978
Unmethylated C's in CpG context 12354095
Unmethylated C's in CHG context 28093062
Unmethylated C's in CHH context 93511997
Percentage methylation (CpG context) 29.1%
Percentage methylation (CHG context) 1.1%
Percentage methylation (CHH context) 1.5%

M-Bias Plot